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BDH2 Monoclonal antibody

BDH2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B6H5

Cat No : 68279-1-Ig

Print datasheet

Synonyms

BDH2, DHRS6, EFA6R, FLJ13261, Oxidoreductase UCPA, PRO20933, SDR15C1, UCPA OR, UNQ6308

验证数据展示

  • WB analysis using 68279-1-Ig

    WB analysis using 68279-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68279-1-Ig (BDH2 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of HeLa using 68279-1-Ig

    WB analysis of HeLa using 68279-1-Ig

    WB result of BDH2 antibody (68279-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells.

  • IF Staining of HepG2 using 68279-1-Ig

    IF Staining of HepG2 using 68279-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BDH2 antibody (68279-1-Ig, Clone: 1B6H5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68279-1-Ig targets BDH2 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human
Immunogen BDH2 fusion protein Ag26108 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 3-hydroxybutyrate dehydrogenase, type 2
Synonyms BDH2, DHRS6, EFA6R, FLJ13261, Oxidoreductase UCPA, PRO20933, SDR15C1, UCPA OR, UNQ6308
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC095414
Gene Symbol BDH2
Gene ID (NCBI) 56898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BUT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytosolic type 2-hydroxybutyrate dehydrogenase, BDH2, originally named DHRS6, plays a physiological role in the cytosolic utilization of ketone bodies, which can subsequently enter mitochondria and the tricarboxylic acid cycle (PMID: 16380372). Moreover, BDH2 catalyzes the production of 2, 3-DHBA during enterobactin biosynthesis and participates in 24p3 (LCN2)-mediated iron transport and apoptosis (PMID: 23150981). It has 3 isoforms with molecular weights of 27, 19, and 16 kDa.

实验方案

Product Specific Protocols
WB protocol for BDH2 antibody 68279-1-IgDownload protocol
IF protocol for BDH2 antibody 68279-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols