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CoraLite® Plus 488-conjugated AIRE Monoclonal antibody

AIRE Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A3C3

Cat No : CL488-66262

Print datasheet

Synonyms

AIRE, AIRE1, APECED, APECED protein, APS1, APSI, autoimmune regulator, PGA1

验证数据展示

  • FC experiment of HeLa using CL488-66262

    FC experiment of HeLa using CL488-66262

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human AIRE (CL488-66262, Clone:1A3C3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AIRE Ready-To-Use IHC Kit. AIRE primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66262-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66262 targets AIRE in FC (Intra) applications and shows reactivity with human, pig samples.

Tested Applications FC (Intra)
Tested Reactivity human, pig
Immunogen AIRE fusion protein Ag19103 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name autoimmune regulator
Synonyms AIRE, AIRE1, APECED, APECED protein, APS1, APSI, autoimmune regulator, PGA1
Calculated Molecular Weight 545 aa, 58 kDa
Observed Molecular Weight 58-69 kDa
GenBank Accession NumberBC137270
Gene Symbol AIRE
Gene ID (NCBI) 326
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43918
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 AIRE antibody CL488-66262Download protocol
Standard Protocols
Click here to view our Standard Protocols