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AIRE Monoclonal antibody

AIRE Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A3C3

Cat No : 66262-1-Ig

Print datasheet

Synonyms

AIRE, AIRE1, APECED, APECED protein, APS1, APSI, autoimmune regulator, PGA1

验证数据展示

  • IF Staining of HeLa using 66262-1-Ig

    IF Staining of HeLa using 66262-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AIRE antibody (66262-1-Ig, Clone: 1A3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 66262-1-Ig

    IF Staining of HeLa using 66262-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AIRE antibody (66262-1-Ig, Clone: 1A3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of human spleen using 66262-1-Ig

    WB analysis of human spleen using 66262-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman spleen tissue, pig spleen tissue
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AIRE Ready-To-Use IHC Kit. AIRE primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66262-1-Ig targets AIRE in WB, IF, ELISA applications and shows reactivity with human, pig samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, pig
Immunogen AIRE fusion protein Ag19103 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name autoimmune regulator
Synonyms AIRE, AIRE1, APECED, APECED protein, APS1, APSI, autoimmune regulator, PGA1
Calculated Molecular Weight 545 aa, 58 kDa
Observed Molecular Weight 58-69 kDa
GenBank Accession NumberBC137270
Gene Symbol AIRE
Gene ID (NCBI) 326
RRIDAB_2881649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43918
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AIRE antibody 66262-1-IgDownload protocol
IF protocol for AIRE antibody 66262-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols