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- KD/KO Validated
AGR2 Polyclonal antibody
AGR2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, rat colon tissue, rat stomach tissue, mouse stomach tissue |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human breast cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy AGR2 Ready-To-Use IHC Kit. AGR2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12275-1-AP targets AGR2 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | AGR2 fusion protein Ag2919 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | anterior gradient homolog 2 (Xenopus laevis) |
| Synonyms | AG 2, AG2, AGR2, GOB 4, HAG 2, HPC8, PDIA17, XAG 2 |
| Calculated Molecular Weight | 175 aa, 20 kDa |
| Observed Molecular Weight | 17-20 kDa |
| GenBank Accession Number | BC015503 |
| Gene Symbol | AGR2 |
| Gene ID (NCBI) | 10551 |
| RRID | AB_2225096 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95994 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used in assessment of high-risk patients undergoing endoscopic procedures.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AGR2 antibody 12275-1-AP | Download protocol |
| IHC protocol for AGR2 antibody 12275-1-AP | Download protocol |
| IP protocol for AGR2 antibody 12275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia. | ||
Nat Protoc Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection. | ||
Proc Natl Acad Sci U S A A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer. | ||
J Pathol A novel blueprint for 'top down' differentiation defines the cervical squamocolumnar junction during development, reproductive life, and neoplasia. | ||
Int J Cancer Unique recurrence patterns of cervical intraepithelial neoplasia following excision of the squamo-columnar junction. | ||
J Proteome Res Identification of serum biomarkers for colorectal cancer metastasis using a differential secretome approach. |
