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人氧化磷酸化免疫印迹试剂盒

宿主

兔

反应种属

人

应用

WB

偶联物

未偶联

Cat No : PK30006

Print datasheet

Validation Data Gallery

  • Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 14676-1-AP (ATP5A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 14676-1-AP (ATP5A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 55070-1-AP (MTCO2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 55070-1-AP (MTCO2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

产品介绍

    OXPHOS（氧化磷酸化）通过线粒体呼吸链将电子传输到分子氧，这涉及四种蛋白复合物。复合物 I、II、III 和 IV（CI、CII、CIII 和 CIV）和两个移动电子载体：辅酶 Q (CoQ) 和细胞色素 c。 呼吸链产生跨膜质子梯度，由复合物 V（也称为 ATP 合酶，CV）引导以合成 ATP。

     此 OXPHOS 试剂盒一管包含五种多克隆抗体，适用于对来自人的线粒体蛋白样本中 5 种 OXPHOS 复合物的相对水平进行蛋白质印迹分析。此试剂盒抗体为五种抗体混合而成。需要检测单独蛋白可按相应货号购置。


Cat.No Name

Complex

MW Size
Antibodies 14676-1-AP ATP5A1 Complexes Ⅴ 50-55 kDa 100ul
21705-1-AP UQCRC1 Complexes Ⅲ 45 kDa
10620-1-AP SDHB Complexes Ⅱ 32 kDa
55070-1-AP MTCO2 Complexes Ⅳ 23-25 kDa
14794-1-AP NDUFB8 ComplexesⅠ 19-22 kDa


保存条件

-20℃保存。自收到之日起一年内保持稳定。

使用方法

Western Blot Protocol


推荐稀释比

APPLICATIONDILUTION
Western Blot (WB)WB : 1:1000-1:8000