Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25871-1-AP (PR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed T-47D cells using PR antibody (25871-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
T-47D cells were subjected to SDS PAGE followed by western blot with 25871-1-AP (PR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25871-1-AP (PR Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).