Various lysates were subjected to SDS PAGE followed by western blot with 13999-1-AP (OLIG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13999-1-AP (OLIG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13999-1-AP (OLIG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 13999-1-AP (OLIG2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP result of anti-OLIG2 (IP:13999-1-AP, 4ug; Detection:13999-1-AP 1:1000) with mouse brain tissue lysate 1600 ug.