Various lysates were subjected to SDS PAGE followed by western blot with 10366-1-AP (Vimentin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WB result of Vimentin antibody (10366-1-AP, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 10366-1-AP (Vimentin antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10366-1-AP (Vimentin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were stained with 0.2ug Vimentin antibody (10366-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (66009-1-Ig, Clone: 2D4H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.

IP result of anti-Beta Actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.

1X10^6 HeLa cells were stained with 0.05 ug Anti-Human Beta Actin (66009-1-Ig, Clone:2D4H5) and FITC-Goat anti-mouse IgG at dilution 1:100 (red), or stained with 0.05 ug isotype control and FITC-Goat anti-mouse IgG at dilution 1:100 (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Immunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Beta Actin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Beta Actin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:102400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WB result of Vimentin antibody (60330-1-Ig, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.

Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60330-1-Ig (Vimentin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

1X10^6 Jurkat cells were stained with 0.2 ug Anti-Human Vimentin (60330-1-Ig, Clone:3H9D1) and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1500 (red), or stained with 0.2 ug isotype control and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1500 (blue). Cells were fixed with 90% MeOH.

Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Vimentin antibody (60330-1-Ig, Clone: 3H9D1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens)..

Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens)..

Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD8 antibody (66868-1-Ig, Clone: 1G2B10, green).

Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD8 antibody (66868-1-Ig, Clone: 1G2B10, green).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human pan-keratin (26411-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Various lysates were subjected to SDS PAGE followed by western blot with 26411-1-AP (pan-keratin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with CL594-66009 (Beta Actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Beta Actin (CL594-66009, Clone:2D4H5) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5 ) at dilution of 1:200.

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5 ) at dilution of 1:200.

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Biotin-60008 (beta Actin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Biotin-60008 (beta Actin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Various lysates were subjected to SDS PAGE followed by western blot with 80762-1-RR (Tubulin-Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

WB result of Alpha Tubulin antibody (80762-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HEK-293 cells.

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-60330 (Vimentin antibody) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Vimentin (CL594-60330, Clone:3H9D1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).