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- KD/KO Validated
CoraLite® Plus 488-conjugated Zyxin Polyclonal antibody
Zyxin Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy ZYX Ready-To-Use IHC Kit. ZYX primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10330-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-10330 targets Zyxin in IF applications and shows reactivity with human samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | human |
| Immunogen | Zyxin fusion protein Ag0419 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | zyxin |
| Synonyms | ESP 2, HED 2, ZYX, zyxin, Zyxin 2 |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 78 kDa |
| GenBank Accession Number | BC008743 |
| Gene Symbol | ZYX |
| Gene ID (NCBI) | 7791 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15942 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Zyxin antibody CL488-10330 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |