• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated ZFP36L1 Monoclonal antibody

ZFP36L1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H6G6

Cat No : CL488-68038

Print datasheet

Synonyms

Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1

验证数据展示

  • IF Staining of HepG2 using CL488-68038

    IF Staining of HepG2 using CL488-68038

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ZFP36L1 antibody (CL488-68038, Clone: 1H6G6 ) at dilution of 1:600, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-68038

    FC experiment of HeLa using CL488-68038

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ZFP36L1 (CL488-68038, Clone:1H6G6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68038-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:300-1:1200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68038 targets ZFP36L1 in IF, FC (Intra) applications and shows reactivity with Human, Rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Rat
Immunogen ZFP36L1 fusion protein Ag30469 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name zinc finger protein 36, C3H type-like 1
Synonyms Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1
Calculated Molecular Weight 338 aa, 36 kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberBC018340
Gene Symbol ZFP36L1
Gene ID (NCBI) 677
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07352
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

As a member of the Tristetraprolin (TTP) RNA binding protein family, ZFP36L1 regulate gene expression post-transcriptionally by promoting mRNA decay. In mammals, ZFP36L1 and its family members have been shown to regulate development, cell diferentiation, cell cycle, apoptosis and anti-cancer capability by targeting an extensive overlapping repertoire of mRNAs.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ZFP36L1 antibody CL488-68038Download protocol
FC protocol for CL Plus 488 ZFP36L1 antibody CL488-68038Download protocol
Standard Protocols
Click here to view our Standard Protocols