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XRN1 Polyclonal antibody

XRN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23108-1-AP

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Synonyms

5 3 exoribonuclease 1, 5' 3' exoribonuclease 1, SEP1, XRN1



经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23108-1-AP targets XRN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen XRN1 fusion protein Ag19398 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 5'-3' exoribonuclease 1
Synonyms 5 3 exoribonuclease 1, 5' 3' exoribonuclease 1, SEP1, XRN1
Calculated Molecular Weight 1706 aa, 194 kDa
Observed Molecular Weight 175 kDa
GenBank Accession NumberBC078146
Gene Symbol XRN1
Gene ID (NCBI) 54464
RRIDAB_2879211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZH2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Exoribonuclease I (XRN1), also known as Sep1 or Rar5, is a 1,694-amino acid protein that functions as the major cytoplasmic 5 prime to 3 prime exoribonuclease and plays an important role in mRNA turnover. XRN1 may also function in the microtubular cytoskeleton as well as in DNA recombination and replication. XRN1 induces the expression of stress granules (SGs), cytoplasmic aggregates of stalled translational preinitiation complexes that accumulate during stress, and GW bodies/processing bodies (PBs), distinct cytoplasmic sites of mRNA degradation. There are several isoforms of XRN1 that are produced as a result of alternative splicing events.Loss of XRN1 markedly affects cell growth rates.This antibody is specific to the XRN1 protein.

实验方案

Product Specific Protocols
WB protocol for XRN1 antibody 23108-1-APDownload protocol
IHC protocol for XRN1 antibody 23108-1-APDownload protocol
IF protocol for XRN1 antibody 23108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Res

NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer

Authors - Ruihui Xie
humanWB

Nucleic Acids Res

LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential

Authors - Damian M Janecki
  • KD Validated