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Vinculin Monoclonal antibody, PBS Only

Vinculin Monoclonal Antibody for WB,IHC,IF,FC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB,IHC,IF,FC,ELISA

Conjugate

Unconjugated

CloneNo.

2B5A7

Cat No : 66305-1-PBS

Print datasheet

Synonyms

CMD1W, Metavinculin, MVCL, VCL, vinculin

验证数据展示

  • WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

    WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

    IF Staining of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66305-1-Ig(Vinculin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

    FC experiment of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

    1X10^6 HeLa cells were stained with 0.2ug Vinculin antibody (66305-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

产品信息

66305-1-PBS targets Vinculin in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB,IHC,IF,FC,ELISA
Tested Reactivity human, mouse, rat, pig
Immunogen Vinculin fusion protein Ag24946 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vinculin
Synonyms CMD1W, Metavinculin, MVCL, VCL, vinculin
Calculated Molecular Weight 1133 aa, 124 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC039174
Gene Symbol VCL
Gene ID (NCBI) 7414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18206
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment.

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.