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- KD/KO Validated
VCP Monoclonal antibody
VCP Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
FC, IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2A4B10
验证数据展示
经过测试的应用
Positive WB detected in | RAW 264.7 cells, WT and VCP KO U2OS cells, SH-SY5Y cells, HeLa cells |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | RAW 264.7 cells, HeLa cells, SH-SY5Y cells |
Positive FC detected in | HL-60 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60316-1-Ig targets VCP in WB, IF, FC, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, ChIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | VCP fusion protein Ag1002 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | valosin-containing protein |
Synonyms | 15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP |
Calculated Molecular Weight | 89 kDa |
Observed Molecular Weight | 89 kDa |
GenBank Accession Number | BC007562 |
Gene Symbol | VCP |
Gene ID (NCBI) | 7415 |
RRID | AB_2881427 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P55072 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for VCP antibody 60316-1-Ig | Download protocol |
IHC protocol for VCP antibody 60316-1-Ig | Download protocol |
IF protocol for VCP antibody 60316-1-Ig | Download protocol |
FC protocol for VCP antibody 60316-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Brain Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.
| ||
Acta Pharmacol Sin Gossypol, a novel modulator of VCP, induces autophagic degradation of mutant huntingtin by promoting the formation of VCP/p97-LC3-mHTT complex. | ||
Biochim Biophys Acta Gene Regul Mech GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1 | ||
Research (Wash D C) SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage |