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CoraLite® Plus 488-conjugated VAPA Monoclonal antibody

VAPA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F10F9

Cat No : CL488-67845

Print datasheet

Synonyms

33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA

验证数据展示

  • FC experiment of HepG2 using CL488-67845

    FC experiment of HepG2 using CL488-67845

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human VAPA (CL488-67845, Clone:1F10F9) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67845-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67845 targets VAPA in FC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC
Tested Reactivity Human, Mouse, Rat
Immunogen VAPA fusion protein Ag7819 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Synonyms 33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC002992
Gene Symbol VAPA
Gene ID (NCBI) 9218
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P0L0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 VAPA antibody CL488-67845Download protocol
Standard Protocols
Click here to view our Standard Protocols