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TLR4 Polyclonal antibody

TLR4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30400-1-AP

Print datasheet

Synonyms

Lps, Ly87, Ran/M1, Rasl2 8, Tlr4, toll like receptor 4

验证数据展示

  • WB analysis using 30400-1-AP

    WB analysis using 30400-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30400-1-AP (TLR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJ774A.1 cells, THP-1 cells, RAW 264.7 cells, mouse liver tissue, mouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30400-1-AP targets TLR4 in WB, ELISA applications and shows reactivity with Mouse, Human samples.

Tested Applications WB, ELISA
Tested Reactivity Mouse, Human
Immunogen TLR4 fusion protein Ag32998 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name toll-like receptor 4
Synonyms Lps, Ly87, Ran/M1, Rasl2 8, Tlr4, toll like receptor 4
Calculated Molecular Weight96 kDa
Observed Molecular Weight96 kDa
GenBank Accession NumberNM_021297
Gene Symbol Tlr4
Gene ID (NCBI) 21898
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9QUK6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TLR4, also known as CD284, belongs to the Toll-like receptor family. It is a type I transmembrane protein consisting of an extracellular leucine-rich repeat region, a helix transmembrane domain, and an intracellular Toll-IL-1 receptor domain. TLR4 functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. It interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP, and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response.

实验方案

Product Specific Protocols
WB protocol for TLR4 antibody 30400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols