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TSG101 Monoclonal antibody, PBS Only

TSG101 Monoclonal Antibody for WB,IHC,IF,FC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,IHC,IF,FC,ELISA

Conjugate

Unconjugated

CloneNo.

2B7G8

Cat No : 67381-1-PBS

Print datasheet

Synonyms

Cell and organelle markers, ESCRT I complex subunit TSG101, Exosome marker, TSG10, TSG101, tumor susceptibility gene 101, VPS23

验证数据展示

  • WB analysis using 67381-1-Ig (same clone as 67381-1-PBS)

    WB analysis using 67381-1-Ig (same clone as 67381-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67381-1-Ig (same clone as 67381-1-PBS)

    IHC staining of human lung cancer using 67381-1-Ig (same clone as 67381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TSG101 antibody included
  • IF Staining of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

    IF Staining of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67381-1-Ig (TSG101 antibody), at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

    FC experiment of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

    1X10^6 HeLa cells were stained with 0.5 ug Anti-Human TSG101 (67381-1-Ig, Clone:2B7G8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • WB analysis of human urine exosomes using 67381-1-Ig (same clone as 67381-1-PBS)

    WB analysis of human urine exosomes using 67381-1-Ig (same clone as 67381-1-PBS)

    human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TSG101 Ready-To-Use IHC Kit. TSG101 primary antibody included.

产品信息

67381-1-PBS targets TSG101 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,IHC,IF,FC,ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen TSG101 fusion protein Ag28569 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor susceptibility gene 101
Synonyms Cell and organelle markers, ESCRT I complex subunit TSG101, Exosome marker, TSG10, TSG101, tumor susceptibility gene 101, VPS23
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 43-46 kDa
GenBank Accession NumberBC002487
Gene Symbol TSG101
Gene ID (NCBI) 7251
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99816
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.