- Featured Product
- KD/KO Validated
CoraLite®594-conjugated TMEM173/STING Polyclonal antibody
TMEM173/STING Polyclonal Antibody for FC (Intra), IF/ICC,IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra), IF/ICC,IF-P
Conjugate
CoraLite®594 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | HT-29 cells, human tonsillitis tissue, HepG2 cells |
| Positive FC detected in | THP-1 cells, HepG2 cells, NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy STING Ready-To-Use IHC Kit. STING primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19851-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-19851 targets TMEM173/STING in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC (Intra), IF/ICC,IF-P |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | TMEM173/STING fusion protein Ag13921 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transmembrane protein 173 |
| Synonyms | FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, STING, TMEM173, transmembrane protein 173 |
| Calculated Molecular Weight | 379 aa, 42 kDa |
| Observed Molecular Weight | 35-40 kDa, 80 kDa |
| GenBank Accession Number | BC047779 |
| Gene Symbol | TMEM173 |
| Gene ID (NCBI) | 340061 |
| RRID | AB_2919854 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86WV6 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 TMEM173/STING antibody CL594-19851 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neuroinflammation The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage |
