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TIP47 Monoclonal antibody, PBS Only

TIP47 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

4C11B1

Cat No : 66523-1-PBS

Print datasheet

Synonyms

47 kDa MPR binding protein, Cargo selection protein TIP47, M6PRBP1, Perilipin 3, Placental protein 17, PLIN3, PP17, TIP47

验证数据展示

  • IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

    IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The F-actin was stained with CL594-Phalloidin (red). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

    IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

    IHC staining of mouse liver using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66523-1-Ig (TIP47 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • WB analysis using 66523-1-Ig (same clone as 66523-1-PBS)

    WB analysis using 66523-1-Ig (same clone as 66523-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 66523-1-Ig (same clone as 66523-1-PBS)

    WB analysis of U2OS using 66523-1-Ig (same clone as 66523-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 66523-1-Ig (TIP47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

    IF Staining of HeLa using 66523-1-Ig (same clone as 66523-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (66523-1-Ig, Clone: 4C11B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66523-1-PBS in a different storage buffer formulation.

产品信息

66523-1-PBS targets TIP47 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human, mouse
Immunogen TIP47 fusion protein Ag1028 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mannose-6-phosphate receptor binding protein 1
Synonyms 47 kDa MPR binding protein, Cargo selection protein TIP47, M6PRBP1, Perilipin 3, Placental protein 17, PLIN3, PP17, TIP47
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC007566
Gene Symbol M6PRBP1
Gene ID (NCBI) 10226
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60664
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.