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ChromoTek TIGIT VHH, recombinant binding protein

Alpaca anti-TIGIT VHH, purified recombinant binding protein. Suitable for conjugation of dyes, biotin, beads, surfaces, etc. via NHS ester reaction

Specificity/Target

TIGIT-Tag

Applications

Conjugation

Type

Nanobody

Conjugate

Unconjugated

Cat No : tgtCys

Synonyms

TIGIT-Nanobody, TIGIT-single domain antibody (sdAb), DKFZp667A205; FLJ39873; VSIG9; VSTM3

Validation Data Gallery

  • The affinity of anti-human TIGIT recombinant VHH towards human TIGIT was determined using biolayer interferometry (BLI). Biotinylated, recombinant human TIGIT was immobilized on Streptavidin biosensors and assayed with 0.15 to 10 nM of CoraLite® Plus 647-conjugated TIGIT VHH (CL647-tgt). Fit indicates a 1:1 binding model fitted to the data.

    The affinity of anti-human TIGIT recombinant VHH towards human TIGIT was determined using biolayer interferometry (BLI). Biotinylated, recombinant human TIGIT was immobilized on Streptavidin biosensors and assayed with 0.15 to 10 nM of CoraLite® Plus 647-conjugated TIGIT VHH (CL647-tgt). Fit indicates a 1:1 binding model fitted to the data.

Product Information

Alpaca anti-TIGIT VHH, purified recombinant binding protein. Suitable for conjugation of dyes, biotin, beads, surfaces, etc. via NHS ester reaction

ApplicationsConjugation
ReactivityHuman
ConjugateUnconjugated
Type Nanobody
ClassRecombinant
HostAlpaca
Affinityin the picomolar range, below the assay limit (biolayer interferometry)
Molecular Weight14.346 kDa
RRID/
Storage Buffer10 mM HEPES, 500 mM NaCl, pH 7.0, 1 mM TCEP
Storage ConditionStore at -20°C; Shipped at dry ice.
BackgroundTIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).