TDP-43 (C-terminal) Recombinant antibody
TDP-43 (C-terminal) Recombinant Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
FC, IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
5D17
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, HSC-T6 cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:2500-1:10000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81350-1-RR targets TDP-43 (C-terminal) in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | fusion protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | TAR DNA binding protein |
Synonyms | ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43 |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 43 kDa |
GenBank Accession Number | BC001487 |
Gene Symbol | TARDBP |
Gene ID (NCBI) | 23435 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13148 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TDP-43 (C-terminal) antibody 81350-1-RR | Download protocol |
IHC protocol for TDP-43 (C-terminal) antibody 81350-1-RR | Download protocol |
IF protocol for TDP-43 (C-terminal) antibody 81350-1-RR | Download protocol |
FC protocol for TDP-43 (C-terminal) antibody 81350-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |