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CoraLite® Plus 488-conjugated TAU Polyclonal antibody

TAU Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10274

Print datasheet

Synonyms

DDPAC, FLJ31424, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU

验证数据展示

  • IF Staining of mouse brain using CL488-10274

    IF Staining of mouse brain using CL488-10274

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 TAU antibody (CL488-10274) at dilution of 1:200.

经过测试的应用

Positive IF detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 10274-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10274 targets TAU in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P
Tested Reactivity human, mouse, rat
Immunogen TAU fusion protein Ag0354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microtubule-associated protein tau
Synonyms DDPAC, FLJ31424, FTDP 17, MAPT, MAPTL, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, PPND, TAU
Calculated Molecular Weight 37-46, 79-81 kDa
Observed Molecular Weight 45-70 kDa, 100 kDa
GenBank Accession NumberBC000558
Gene Symbol MAPT
Gene ID (NCBI) 4137
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10636
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Present polyclonal anti-Tau antibody was produced by immunizing animals with N-terminal of Tau and can detect approx 45-70 kDa and 100-kDa bands in brain tissues.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TAU antibody CL488-10274Download protocol
Standard Protocols
Click here to view our Standard Protocols