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Band 3 / AE1 Recombinant antibody

Band 3 / AE1 Recombinant Antibody for IF-P, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF-P, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3K21

Cat No : 81066-1-RR

Print datasheet

Synonyms

AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, Band 3 anion transport protein, BND3, CD233, DI, EMPB3, EPB3, FR, RTA1A, SLC4A1, SW, WD, WD1, WR

验证数据展示

  • IF Staining of human placenta using 81066-1-RR

    IF Staining of human placenta using 81066-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human placenta using 81066-1-RR

    IF Staining of human placenta using 81066-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human placenta using 81066-1-RR

    IHC staining of human placenta using 81066-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Band 3/AE1 antibody included

经过测试的应用

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Band 3/AE1 Ready-To-Use IHC Kit. Band 3/AE1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81066-1-RR targets Band 3 / AE1 in IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF-P, IHC, ELISA
Tested Reactivity Human
Immunogen Band 3 / AE1 fusion protein Ag27878 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Synonyms AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, Band 3 anion transport protein, BND3, CD233, DI, EMPB3, EPB3, FR, RTA1A, SLC4A1, SW, WD, WD1, WR
Calculated Molecular Weight 911 aa, 102 kDa
GenBank Accession NumberBC101570
Gene Symbol SLC4A1
Gene ID (NCBI) 6521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02730
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.

实验方案

Product Specific Protocols
IHC protocol for Band 3 / AE1 antibody 81066-1-RRDownload protocol
IF protocol for Band 3 / AE1 antibody 81066-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols