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Band 3/ AE1 Polyclonal antibody

Band 3/ AE1 Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

IF-P, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 28131-1-AP

Print datasheet

Synonyms

AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, band 3, Band 3 anion transport protein, band 3/ AE1, CD233, SLC4A1

验证数据展示

  • WB analysis of mouse liver using 28131-1-AP

    WB analysis of mouse liver using 28131-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 28131-1-AP

    WB analysis of COLO 320 using 28131-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 28131-1-AP

    IHC staining of human spleen using 28131-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28131-1-AP (SLC4A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Band 3/AE1 antibody included
  • IF Staining of human placenta using 28131-1-AP

    IF Staining of human placenta using 28131-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using band 3/ AE1 antibody (28131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCOLO 320 cells, mouse liver tissue
Positive IHC detected inhuman spleen tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Band 3/AE1 Ready-To-Use IHC Kit. Band 3/AE1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28131-1-AP targets Band 3/ AE1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse
Immunogen Band 3/ AE1 fusion protein Ag27878 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Synonyms AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, band 3, Band 3 anion transport protein, band 3/ AE1, CD233, SLC4A1
Calculated Molecular Weight 911 aa, 102 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC101570
Gene Symbol SLC4A1
Gene ID (NCBI) 6521
RRIDAB_2881069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02730
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.

实验方案

Product Specific Protocols
WB protocol for Band 3/ AE1 antibody 28131-1-APDownload protocol
IHC protocol for Band 3/ AE1 antibody 28131-1-APDownload protocol
IF protocol for Band 3/ AE1 antibody 28131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.

Authors - Eunus S Ali
View Article
humanIP

Int J Hematol

Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia.

Authors - Akihito Kitao
View Article
mouseIF

eLife

Piezo1 as a force-through-membrane sensor in red blood cells

Authors - George Vaisey
View Article
humanIF

NPJ Regen Med

Ferric citrate and apo-transferrin enable erythroblast maturation with β-globin from hemogenic endothelium

Authors - Soo-Been Jeon
View Article