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SF3B3 Polyclonal antibody

SF3B3 Polyclonal Antibody for IF/ICC,IF-P, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF/ICC,IF-P, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 14577-1-AP

Print datasheet

Synonyms

KIAA0017, RSE1, SAP 130, SAP130, SF3b130, SF3B3, Splicing factor 3B subunit 3, STAF130

验证数据展示

  • WB analysis using 14577-1-AP

    WB analysis using 14577-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 14577-1-AP

    IHC staining of human gliomas using 14577-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14577-1-AP (SF3B3 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with SF3B3 antibody included
  • IF Staining of HeLa using 14577-1-AP

    IF Staining of HeLa using 14577-1-AP

    Immunofluorescent analysis of Hela cells, using SF3B3 antibody 14577-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of rat brain using 14577-1-AP

    IP experiment of rat brain using 14577-1-AP

    IP result of anti-SF3B3 (IP:14577-1-AP, 4ug; Detection:14577-1-AP 1:5000) with rat brain tissue lysate 4800ug.

  • IHC staining of human lung cancer using 14577-1-AP

    IHC staining of human lung cancer using 14577-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SF3B3 antibody included
  • IHC staining of human lung cancer using 14577-1-AP

    IHC staining of human lung cancer using 14577-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SF3B3 antibody included
  • IHC staining of rat brain using 14577-1-AP

    IHC staining of rat brain using 14577-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SF3B3 antibody included
  • IF Staining of mouse heart using 14577-1-AP

    IF Staining of mouse heart using 14577-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SF3B3 antibody (14577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse heart using 14577-1-AP

    IF Staining of mouse heart using 14577-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SF3B3 antibody (14577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, mouse heart tissue, human brain tissue, rat brain tissue, rat heart tissue, HeLa cells
Positive IP detected inrat brain tissue
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, mouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy SF3B3 Ready-To-Use IHC Kit. SF3B3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14577-1-AP targets SF3B3 in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC,IF-P, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, RIP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen SF3B3 fusion protein Ag5980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor 3b, subunit 3, 130kDa
Synonyms KIAA0017, RSE1, SAP 130, SAP130, SF3b130, SF3B3, Splicing factor 3B subunit 3, STAF130
Calculated Molecular Weight 136 kDa
Observed Molecular Weight130-135 kDa
GenBank Accession NumberBC003146
Gene Symbol SF3B3
Gene ID (NCBI) 23450
RRIDAB_2270189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15393
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.

实验方案

Product Specific Protocols
WB protocol for SF3B3 antibody 14577-1-APDownload protocol
IHC protocol for SF3B3 antibody 14577-1-APDownload protocol
IF protocol for SF3B3 antibody 14577-1-APDownload protocol
IP protocol for SF3B3 antibody 14577-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Gastroenterology

Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.

Authors - Yingying Cao
View Article

Genome Res

RNA structure replaces the need for U2AF2 in splicing.

Authors - Chien-Ling Lin
View Article
humanWB

Nat Commun

Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.

Authors - Teng Teng
View Article
humanRIP

Nucleic Acids Res

Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.

Authors - Ruibing Chen
View Article
humanWB

Mol Neurodegener

Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Authors - Qingyang Zhang
View Article
humanWB

Nucleic Acids Res

Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.

Authors - Ruibing Chen
View Article