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SALL4 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

IP, WB

Cat No : 39957,39958 39957

Synonyms

SALL4, Sal-like protein 4, zinc-finger transcription factor, tfab, embryonic stem cell pluripotency, esc, Oct4, IVIC, Duane-radial ray, stem cell, stem cells, stem cell, stem cells, polyclonal, pab, immunoprecipitation, ip, western blotting, wb, antibody, antibodies, sample

验证数据展示

  • SALL4 antibody (pAb) tested by Immunoprecipitation. 10 ul of SALL4 antibody was used to immunoprecipitate SALL4 from 500 ug of F9 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SALL4 antibody at a dilution of 1:500.

    SALL4 antibody (pAb) tested by Immunoprecipitation. 10 ul of SALL4 antibody was used to immunoprecipitate SALL4 from 500 ug of F9 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SALL4 antibody at a dilution of 1:500.

  • SALL4 antibody (pAb) tested by Western blot. Nuclear extract of P19 cells (20 μg) probed with SALL4 antibody (1:500).

    SALL4 antibody (pAb) tested by Western blot. Nuclear extract of P19 cells (20 μg) probed with SALL4 antibody (1:500).

产品信息

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ul per IP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This SALL4 antibody was raised against a synthetic peptide derived from the N-terminus of human SALL4.
Full Name SALL4 antibody (pAb)
Synonyms SALL4, Sal-like protein 4, zinc-finger transcription factor, tfab, embryonic stem cell pluripotency, esc, Oct4, IVIC, Duane-radial ray, stem cell, stem cells, stem cell, stem cells, polyclonal, pab, immunoprecipitation, ip, western blotting, wb, antibody, antibodies, sample
Molecular weight 142 kDa
GenBank accession numberNP_065169
RRIDAB_2793408
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

SALL4 (Sal-like protein 4) is a zinc-finger transcription factor required for the maintenance of embryonic stem cell pluripotency. SALL4 activates the expression of Oct4 and reduction in the levels of SALL4 eliminates the ability of ES cells to self-renew and causes differentiation. SALL4 mutations are associated with the developmental disorders IVIC syndrome and Duane-radial ray syndrome.