Recombinant METTL3 protein

Recombinant METTL3 protein is supplied at a concentration of 0.35 ug/ul in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.

N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also occurs in other RNA molecules, such as primary miRNA (pri-miRNAs). METTL3 (methyltransferase-like 3, also known as IME4, M6A, MT-A70) forms a stable N6-methyltransferase heterodimer complex with METTL14, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition. METTL3 promotes translation by interacting with the translation initiation machinery in the cytoplasm. Its overexpression in a number of cancer cells suggests that it may participate to cancer cell proliferation by promoting mRNA translation.

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Recombinant METTL3 protein was expressed in a Baculovirus expression system as the full length protein (accession number NP_062826.2) with an N-terminal FLAG-Tag. The molecular weight of METTL3 is 65.5 kDa.

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.