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- KD/KO Validated
PTPN11/SHP2 Polyclonal antibody
PTPN11/SHP2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, A549 cells, human brain tissue, human heart tissue, Jurkat cells, MCF-7 cells, NIH/3T3 cells, U-937 cells, HEK-293 cells, HeLa cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human breast cancer tissue, human gliomas tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20145-1-AP targets PTPN11/SHP2 in WB, IP, IF, IHC, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC, CoIP, Cell treatment |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | PTPN11/SHP2 fusion protein Ag13660 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein tyrosine phosphatase, non-receptor type 11 |
Synonyms | BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2 |
Calculated Molecular Weight | 597 aa, 68 kDa |
Observed Molecular Weight | 68-75 kDa |
GenBank Accession Number | BC008692 |
Gene Symbol | PTPN11 |
Gene ID (NCBI) | 5781 |
RRID | AB_10699877 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q06124 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PTPN11/SHP2 antibody 20145-1-AP | Download protocol |
IHC protocol for PTPN11/SHP2 antibody 20145-1-AP | Download protocol |
IF protocol for PTPN11/SHP2 antibody 20145-1-AP | Download protocol |
IP protocol for PTPN11/SHP2 antibody 20145-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Hepatology Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.
| ||
Oncogene Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis. | ||
Cell Chem Biol Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells. | ||
Mol Ther Nucleic Acids miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance. | ||
Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. | ||
Aging (Albany NY) Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells. |