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CoraLite®594-conjugated PSMA/GCPII Monoclonal antibody

PSMA/GCPII Monoclonal Antibody for FC (Intra), IF/ICC,IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

FC (Intra), IF/ICC,IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G4E12

Cat No : CL594-66678

Print datasheet

Synonyms

FGCP, Folate hydrolase 1, FOLH, FOLH1, GCP2, GCPII, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, mGCP, NAALAD1, NAALAdase, NAALADase I, PSM, PSMA, PSMA/GCPII

验证数据展示

  • IF Staining of human prostate cancer using CL594-66678

    IF Staining of human prostate cancer using CL594-66678

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite®594 PSMA/GCPII antibody (CL594-66678, Clone: 3G4E12 ) at dilution of 1:200.

  • IF Staining of PC-3 using CL594-66678

    IF Staining of PC-3 using CL594-66678

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite®594 PSMA/GCPII antibody (CL594-66678, Clone: 3G4E12 ) at dilution of 1:200.

  • FC experiment of LNCaP using CL594-66678

    FC experiment of LNCaP using CL594-66678

    1X10^6 LNCaP cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PSMA/GCPII (CL594-66678, Clone:3G4E12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inPC-3 cells, human prostate cancer tissue
Positive FC detected inLNCaP cells
For other applications, we recommend the unconjugated version of this antibody, 66678-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66678 targets PSMA/GCPII in IF, FC (Intra) applications and shows reactivity with Human, Rat samples.

Tested Applications FC (Intra), IF/ICC,IF-P
Tested Reactivity Human, Rat
Immunogen PSMA/GCPII fusion protein Ag16594 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name folate hydrolase (prostate-specific membrane antigen) 1
Synonyms FGCP, Folate hydrolase 1, FOLH, FOLH1, GCP2, GCPII, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, mGCP, NAALAD1, NAALAdase, NAALADase I, PSM, PSMA, PSMA/GCPII
Calculated Molecular Weight 719 aa, 81 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC025672
Gene Symbol FOLH1
Gene ID (NCBI) 2346
RRIDAB_2920020
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ04609
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL594 PSMA/GCPII antibody CL594-66678Download protocol
Standard Protocols
Click here to view our Standard Protocols