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PPP1CC Monoclonal antibody

PPP1CC Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G11C3

Cat no : 68122-1-Ig

Print datasheet

Synonyms

PP 1G, PPP1CC, PPP1G

验证数据展示

  • WB analysis using 68122-1-Ig

    WB analysis using 68122-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68122-1-Ig (PPP1CC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of C2C12 using 68122-1-Ig

    IF Staining of C2C12 using 68122-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using PPP1CC antibody (68122-1-Ig, Clone: 2G11C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 68122-1-Ig

    IF Staining of HeLa using 68122-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PPP1CC antibody (68122-1-Ig, Clone: 2G11C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells, C2C12 cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CC Ready-To-Use IHC Kit. PPP1CC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68122-1-Ig targets PPP1CC in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PPP1CC fusion protein Ag32348 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein phosphatase 1, catalytic subunit, gamma isoform
Synonyms PP 1G, PPP1CC, PPP1G
Calculated Molecular Weight 35 kDa
Observed Molecular Weight35kDa
GenBank Accession NumberBC014073
Gene Symbol PPP1CC
Gene ID (NCBI) 5501
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36873
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

实验方案

Product Specific Protocols
WB protocol for PPP1CC antibody 68122-1-IgDownload protocol
IF protocol for PPP1CC antibody 68122-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols