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CoraLite® Plus 488-conjugated PLDN Monoclonal antibody

PLDN Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4F4C11

Cat No : CL488-66558

Print datasheet

Synonyms

PA, PALLID, Pallid protein homolog, Pallidin, pallidin homolog (mouse), PLDN

验证数据展示

  • IF Staining of MCF-7 using CL488-66558

    IF Staining of MCF-7 using CL488-66558

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 PLDN antibody (CL488-66558, Clone: 4F4C11 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66558-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66558 targets PLDN in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen PLDN fusion protein Ag18095 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pallidin homolog (mouse)
Synonyms PA, PALLID, Pallid protein homolog, Pallidin, pallidin homolog (mouse), PLDN
Calculated Molecular Weight 23~24 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC004819
Gene Symbol PLDN
Gene ID (NCBI) 26258
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UL45
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pallidin, also named as PLDN and PA, is involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. It may play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process. Pallidin interacts with syntaxin-12 which is a t-SNARE protein that mediates vesicle docking and fusion.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PLDN antibody CL488-66558Download protocol
Standard Protocols
Click here to view our Standard Protocols