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  • Featured Product
  • KD/KO Validated

PLAP Monoclonal antibody, PBS Only

PLAP Monoclonal Antibody for WB,Indirect ELISA,IHC,FC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,Indirect ELISA,IHC,FC

Conjugate

Unconjugated

CloneNo.

2B7C9

Cat No : 60294-1-PBS

Print datasheet

Synonyms

ALP, ALPP, PALP, Placental alkaline phosphatase, PLAP, PLAP 1

验证数据展示

  • WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    WB result of PLAP antibody (60294-1-Ig, 1:1000) with si-control and si-PLAP transfected HepG2 cells. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    FC experiment of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human PLAP (60294-1-Ig, Clone:2B7C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALPP/PLAP antibody included
  • IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALPP/PLAP antibody included
  • WB analysis using 60294-1-Ig (same clone as 60294-1-PBS)

    WB analysis using 60294-1-Ig (same clone as 60294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ALPP/PLAP Ready-To-Use IHC Kit. ALPP/PLAP primary antibody included.

产品信息

60294-1-PBS targets PLAP in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC,FC
Tested Reactivity human
Immunogen PLAP fusion protein Ag13361 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alkaline phosphatase, placental (Regan isozyme)
Synonyms ALP, ALPP, PALP, Placental alkaline phosphatase, PLAP, PLAP 1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC009647
Gene Symbol ALPP
Gene ID (NCBI) 250
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05187
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.