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CoraLite®555-conjugated PEX5 Polyclonal antibody
PEX5 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 12545-1-AP
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-12545 targets PEX5 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra) |
Tested Reactivity | human, mouse, rat |
Immunogen | PEX5 fusion protein Ag3268 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | peroxisomal biogenesis factor 5 |
Synonyms | FLJ50634, FLJ50721, FLJ51948, Peroxin 5, Peroxisome Marker, Peroxisome receptor 1, PEX5, PTS1 BP, PTS1 receptor, PTS1R, PXR1 |
Calculated Molecular Weight | 631 aa, 70 kDa |
Observed Molecular Weight | 68-80 kDa |
GenBank Accession Number | BC010621 |
Gene Symbol | PEX5 |
Gene ID (NCBI) | 5830 |
RRID | AB_2919611 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570nm |
Form | Liquid |
Purification Method | Antigen Affinity Purified |
UNIPROT ID | P50542 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder. This antibody recognizes endogenous PEX5, which migrates with an apparent molecular mass of 80 kDa (PMID: 7790377).
实验方案
Product Specific Protocols | |
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FC protocol for CL555 PEX5 antibody CL555-12545 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |