CoraLite® Plus 488-conjugated PEDF Monoclonal antibody
PEDF Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
Human, pig
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2C5C8
验证数据展示
经过测试的应用
Positive WB detected in | pig plasma, human plasma |
Planning an IHC experiment? We recommend our IHCeasy SERPINF1/PEDF Ready-To-Use IHC Kit. SERPINF1/PEDF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66554-1-Ig
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66554 targets PEDF in WB applications and shows reactivity with Human, pig samples.
Tested Applications | WB |
Tested Reactivity | Human, pig |
Immunogen | PEDF fusion protein Ag26660 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 |
Synonyms | EPC 1, PEDF, PIG35, Serpin F1, SERPINF1 |
Calculated Molecular Weight | 418 aa, 46 kDa |
Observed Molecular Weight | 46-50 kDa |
GenBank Accession Number | BC013984 |
Gene Symbol | SERPINF1 |
Gene ID (NCBI) | 5176 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P36955 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.
实验方案
Product Specific Protocols | |
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WB protocol for CL Plus 488 PEDF antibody CL488-66554 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |