CoraLite® Plus 647 Anti-Human PD-L1 (B7-H1) (29E.2A3)
PD-L1 (B7-H1) Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
29E.2A3
验证数据展示
经过测试的应用
| Positive FC detected in | human peripheral blood lymphocytes |
For other applications, we recommend the unconjugated version of this antibody, 65081-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-65081 targets PD-L1 (B7-H1) in FC applications and shows reactivity with Human samples.
| Tested Applications | FC |
| Tested Reactivity | Human |
| Immunogen | Full length human PD-L1 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD274 molecule |
| Synonyms | B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed death ligand 1 |
| GenBank Accession Number | BC069381 |
| Gene Symbol | CD274 |
| Gene ID (NCBI) | 29126 |
| RRID | AB_2920250 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q9NZQ7 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. |
背景介绍
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 PD-L1 (B7-H1) antibody CL647-65081 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
