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ORAI1 Monoclonal antibody, PBS Only

ORAI1 Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

3C12F9

Cat No : 66223-1-PBS

Print datasheet

Synonyms

ORAI1

验证数据展示

  • WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

  • WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    WB analysis using 66223-1-Ig (same clone as 66223-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 66223-1-Ig (same clone as 66223-1-PBS)

    IF Staining of Jurkat using 66223-1-Ig (same clone as 66223-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI1 antibody (66223-1-Ig, Clone: 3C12F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

产品信息

66223-1-PBS targets ORAI1 in WB, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB,Indirect ELISA,IF
Tested Reactivity human, mouse, rat, pig
Immunogen ORAI1 fusion protein Ag18050 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ORAI calcium release-activated calcium modulator 1
Synonyms ORAI1
Calculated Molecular Weight 301 aa, 33 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC015369
Gene Symbol ORAI1
Gene ID (NCBI) 84876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96D31
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 32-55 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.