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IkB Beta Polyclonal antibody

IkB Beta Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12660-1-AP

Print datasheet

Synonyms

I kappa B beta, IkappaBbeta, IkB B, IkB beta, IKBB, NF kappa B inhibitor beta, NF kappa BIB, NFKBIB, TR interacting protein 9, TRIP 9, TRIP9

验证数据展示

  • WB analysis using 12660-1-AP

    WB analysis using 12660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 12660-1-AP

    IHC staining of human colon using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 12660-1-AP

    IHC staining of human colon using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 12660-1-AP

    IF Staining of HeLa using 12660-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12660-1-AP (IkB beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman colon tissue, human endometrial cancer tissue, human colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12660-1-AP targets IkB Beta in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen IkB Beta fusion protein Ag3340 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Synonyms I kappa B beta, IkappaBbeta, IkB B, IkB beta, IKBB, NF kappa B inhibitor beta, NF kappa BIB, NFKBIB, TR interacting protein 9, TRIP 9, TRIP9
Calculated Molecular Weight 356 aa, 38 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC015528
Gene Symbol NFKBIB
Gene ID (NCBI) 4793
RRIDAB_2151611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IkB Beta antibody 12660-1-APDownload protocol
IHC protocol for IkB Beta antibody 12660-1-APDownload protocol
IF protocol for IkB Beta antibody 12660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Toxicol Appl Pharmacol

Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.

Authors - Azubuike Peter Ebokaiwe
View Article
mouseWB

Gut Microbes

Gut microbiota regulates host melatonin production through epithelial cell MyD88

Authors - Bingnan Liu
View Article