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- KD/KO Validated
NF-κB p65 Recombinant antibody
NF-κB p65 Recombinant Antibody for FC, IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (4)
Applications
FC, IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
4C7
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, MOLT-4 cells, Jurkat cells, Raji cells, NIH/3T3 cells, HSC-T6 cells |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:40000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80979-1-RR targets NF-κB p65 in WB, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | FC, IF/ICC, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | NF-κB p65 fusion protein Ag1199 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | v-rel reticuloendotheliosis viral oncogene homolog A (avian) |
Synonyms | NF κB, NFKB3, NF-κB p65, p65, RELA, Transcription factor p65 |
Calculated Molecular Weight | 65 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC011603 |
Gene Symbol | RELA |
Gene ID (NCBI) | 5970 |
RRID | AB_2918923 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q04206 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NF-κB p65 antibody 80979-1-RR | Download protocol |
IF protocol for NF-κB p65 antibody 80979-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Redox Biol Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice | ||
Int J Biol Macromol A20 ameliorates Aspergillus fumigatus keratitis by promoting autophagy and inhibiting NF-κB signaling | ||
Int J Biol Macromol Angelica sinensis aboveground part polysaccharide and its metabolite 5-MT ameliorate colitis via modulating gut microbiota and TLR4/MyD88/NF-κB pathway | ||
Oxid Med Cell Longev Silencing of Angiopoietin-Like Protein 4 (Angptl4) Decreases Inflammation, Extracellular Matrix Degradation, and Apoptosis in Osteoarthritis via the Sirtuin 1/NF-κB Pathway | ||
J Agric Food Chem A Flower-like Brain Targeted Selenium Nanocluster Lowers the Chlorogenic Acid Dose for Ameliorating Cognitive Impairment in APP/PS1 Mice | ||
Biochem Pharmacol Inhibition of S100A9 alleviates neurogenic pulmonary edema after subarachnoid hemorrhage |