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  • Featured Product
  • KD/KO Validated

NF-κB p65 Recombinant antibody, PBS Only

NF-κB p65 Recombinant Antibody for WB,Indirect ELISA,IF,FC

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IF,FC

Conjugate

Unconjugated

CloneNo.

4C7

Cat No : 80979-1-PBS

Print datasheet

Synonyms

NF κB, NFKB3, NF-κB p65, p65, RELA, Transcription factor p65

验证数据展示

  • WB analysis using 80979-1-RR (same clone as 80979-1-PBS)

    WB analysis using 80979-1-RR (same clone as 80979-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80979-1-RR (NF-κB p65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 80979-1-RR (same clone as 80979-1-PBS)

    WB analysis of HEK-293 using 80979-1-RR (same clone as 80979-1-PBS)

    WB result of NF-κB p65 antibody (80979-1-RR; 1:13000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NF-κB p65 transfected HEK-293 cells. This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80979-1-RR (same clone as 80979-1-PBS)

    IF Staining of HepG2 using 80979-1-RR (same clone as 80979-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using p65; RELA antibody (80979-1-RR, Clone: 4C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 80979-1-RR (same clone as 80979-1-PBS)

    FC experiment of HepG2 using 80979-1-RR (same clone as 80979-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NF-κB p65 (80979-1-RR, Clone:4C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80979-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NF-κB p65/RELA Ready-To-Use IHC Kit. NF-κB p65/RELA primary antibody included.

产品信息

80979-1-PBS targets NF-κB p65 in WB, IF, FC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IF,FC
Tested Reactivity Human, Mouse, Rat
Immunogen NF-κB p65 fusion protein Ag1199 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Synonyms NF κB, NFKB3, NF-κB p65, p65, RELA, Transcription factor p65
Calculated Molecular Weight 65 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC011603
Gene Symbol RELA
Gene ID (NCBI) 5970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04206
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Nuclear factor kB (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.