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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NDP52 Polyclonal antibody

NDP52 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-12229

Print datasheet

Synonyms

CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52

验证数据展示

  • IF Staining of HeLa using CL488-12229

    IF Staining of HeLa using CL488-12229

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 NDP52 antibody (CL488-12229) at dilution of 1:200.

  • FC experiment of HeLa using CL488-12229

    FC experiment of HeLa using CL488-12229

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NDP52 (CL488-12229) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12229-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12229 targets NDP52 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen NDP52 fusion protein Ag2866 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium binding and coiled-coil domain 2
Synonyms CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52
Calculated Molecular Weight 446 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015893
Gene Symbol CALCOCO2
Gene ID (NCBI) 10241
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13137
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NDP52 antibody CL488-12229Download protocol
FC protocol for CL Plus 488 NDP52 antibody CL488-12229Download protocol
Standard Protocols
Click here to view our Standard Protocols