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MultiProTM 5CFLX Anti-Human IBA1 (Polyclonal)

IBA1 Polyclonal Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

GGATAGGTGCCGACA

Cat No : G10904-1-5C

Print datasheet

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

验证数据展示

  • Single Cell Sequencing experiment G10904-1-5C on Resting PBMC

    Single Cell Sequencing experiment G10904-1-5C on Resting PBMC

    G10904-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G10904-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G10904-1-5C on PBMC treated with 4hr LPS + TI

    G10904-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G10904-1-5C targets IBA1 in applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra)
Tested Reactivity Human
Immunogen IBA1 fusion protein Ag1363 种属同源性预测
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Polyclonal
Full Name MultiProTM 5CFLX Anti-Human IBA1 (Polyclonal)
Synonyms AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1
Calculated Molecular Weight 17 kDa
GenBank Accession NumberBC009474
Gene Symbol AIF1
Gene ID (NCBI) 199
ENSEMBL Gene IDENSG00000204472
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGGGATAGGTGCCGACACCCATATAAGAAA
Barcode SequenceGGATAGGTGCCGACA
Form Liquid
UNIPROT IDP55008
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

背景介绍

What is the molecular weight of IBA1?

The molecular weight of IBA1 is 16.7 kD.


 What is IBA1?

Ionized calcium-binding adaptor molecule 1 (IBA1), also known as Allograft inflammatory factor-1 (AIF-1), is an inflammation-responsive scaffold protein expressed and secreted from macrophages. Microglia response factor (MRF-1) and daintain are also similar, and likely identical, proteins (PMIDs: 29749461, 9630473, 23792284).


 What is the function of IBA1?

IBA1 is necessary for macrophage survival, and it is also a key molecule in proinflammatory activity (PMID: 29749461).


 Where is IBA1 localized?

IBA1 is a cytoplasmic protein, often expressed in immunocytes, macrophages, and microglia. It can be used as a marker for normal (not 'dark') microglia in brain tissue, as IBA1 is expressed by all microglial cell subpopulations (PMIDs: 29749461, 11943136, 26847266, 9630473).


 Is IBA1 upregulated in active immunophages?

Yes; its expression is associated with inflammatory activity (PMID: 29749461).


 How do IBA1-positive microglia differ from microglia that express less or no IBA1?

'Dark' microglia is a recently described phenotype associated with Alzheimer's disease pathology and chronic stress; these dark microglia express decreased IBA1 (often punctiform), and show distinct ultrastructural differences from 'normal' microglia as well as condensed and electron-dense cytoplasm and nucleoplasm. Normal microglia generally display strong, diffuse expression of IBA1. IBA1-positive microglial processes in normal conditions also have a strong tendency to exclusively contact synaptic elements, such as axon terminals, dendritic spines, and synaptic clefts (PMIDs: 29992181, 26847266).


 How is IBA1 related to Alzheimer's disease and pain?

Exacerbated immunoactivity of IBA1, particularly in proximity to amyloid plaques, is prominently featured in AD pathology. IBA1, along with other microglial markers, are also associated with pain, and robust microglial reactions often follow spinal cord injury (PMIDs: 29992181, 23792284).


Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol