Leptin Polyclonal antibody
Leptin Polyclonal Antibody for IF/ICC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IHC detected in | human stomach tissue, human liver tissue, human ovary tissue, human placenta tissue, human testis tissue, mouse ovary tissue, mouse brain tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy Leptin Ready-To-Use IHC Kit. Leptin primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 3 publications below |
产品信息
The immunogen of 17436-1-AP is Leptin Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, IHC, ELISA |
| Cited Applications | IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | Leptin fusion protein Ag11507 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | leptin |
| Synonyms | LEP, leptin, OB, Obese protein, Obesity factor, OBS |
| Calculated Molecular Weight | 167 aa, 19 kDa |
| GenBank Accession Number | BC060830 |
| Gene Symbol | LEP |
| Gene ID (NCBI) | 3952 |
| RRID | AB_2265702 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41159 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Leptin is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. Leptin is the most critical hormone in the homeostatic regulation of energy balance among those so far discovered. Leptin primarily acts on the neurons of the mediobasal part of hypothalamus to regulate food intake, thermogenesis, and the blood glucose level. Leptin also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Leptin antibody 17436-1-AP | Download protocol |
| IF protocol for Leptin antibody 17436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomedicines Leptin, Leptin Receptor, KHDRBS1 (KH RNA Binding Domain Containing, Signal Transduction Associated 1), and Adiponectin in Bone Metastasis from Breast Carcinoma: An Immunohistochemical Study. | ||
Mol Immunol Inflammatory arthritis increases the susceptibility to acute immune-mediated hepatitis in mice through enhancing leptin expression in T cells | ||
Biomed Res Int Polydatin Inhibits Adipose Tissue Inflammation and Ameliorates Lipid Metabolism in High-Fat-Fed Mice. | ||
J Immunol Leptin Promotes Monosodium Urate Crystal-Induced Inflammation in Human and Murine Models of Gout. |
