Cytokeratin 18 Recombinant antibody
Cytokeratin 18 Recombinant Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
4F2
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, A549 cells, HCT 116 cells, HepG2 cells, K-562 cells, mouse liver tissue, rat liver tissue |
Positive IHC detected in | human appendicitis tissue, human liver tissue, human renal cell carcinoma tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82817-5-RR targets Cytokeratin 18 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IHC, WB, ELISA |
Tested Reactivity | Human, mouse, rat |
Immunogen | Cytokeratin 18 fusion protein Ag1260 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | keratin 18 |
Synonyms | CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 48 kDa |
GenBank Accession Number | BC000180 |
Gene Symbol | KRT18 |
Gene ID (NCBI) | 3875 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P05783 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Cytokeratin 18 antibody 82817-5-RR | Download protocol |
IHC protocol for Cytokeratin 18 antibody 82817-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |