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CoraLite®555-conjugated KI67 Polyclonal antibody
KI67 Polyclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite®555 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF detected in | HeLa cells |
Positive FC detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 27309-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of CL555-27309 is KI67 Fusion Protein expressed in E. coli.
Tested Applications | FC (Intra), IF/ICC |
Tested Reactivity | human |
Immunogen | KI67 fusion protein Ag26266 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | antigen identified by monoclonal antibody Ki-67 |
Synonyms | Antigen KI 67, Ki 67, KI67, KIA, MKI67 |
Calculated Molecular Weight | 359 kDa |
GenBank Accession Number | NM_002417 |
Gene Symbol | MKI67 |
Gene ID (NCBI) | 4288 |
RRID | AB_2919649 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.
实验方案
Product Specific Protocols | |
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IF protocol for CL555 KI67 antibody CL555-27309 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |