IHCeasy GCK Ready-To-Use IHC Kit
GCK Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC1584
Synonyms
GCK, GK, GLK, Glucokinase, glucokinase (hexokinase 4), Hexokinase 4, Hexokinase D, Hexokinase type IV, HHF3, HK IV, HK4, HKIV, HXKP, MODY2
Validation Data Gallery
Product Information
KHC1584 is a ready-to-use IHC kit for staining of GCK. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy GCK Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism. The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme. Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3).
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | GCK, GK, GLK, Glucokinase, glucokinase (hexokinase 4), Hexokinase 4, Hexokinase D, Hexokinase type IV, HHF3, HK IV, HK4, HKIV, HXKP, MODY2 |