IDH1 Monoclonal antibody
IDH1 Monoclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
CloneNo.
2A6A2
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HeLa cells, DU 145 cells, MCF-7 cells |
Positive IHC detected in | human pancreas tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
66197-1-Ig targets IDH1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, IHC, WB,ELISA |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | IDH1 fusion protein Ag19293 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | isocitrate dehydrogenase 1 (NADP+), soluble |
Synonyms | IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD |
Calculated Molecular Weight | 414 aa, 47 kDa |
Observed Molecular Weight | 46 kDa |
GenBank Accession Number | BC012846 |
Gene Symbol | IDH1 |
Gene ID (NCBI) | 3417 |
RRID | AB_2881590 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O75874 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for IDH1 antibody 66197-1-Ig | Download protocol |
IHC protocol for IDH1 antibody 66197-1-Ig | Download protocol |
IF protocol for IDH1 antibody 66197-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Neurosci TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates. | ||
J Immunol Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues |