Human TGF-beta1 ELISA Kit6 Publications

Catalog number: KE00002



96 T


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Product name:
Human TGF-beta1 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant, Urine

Assay type:

3.3 pg/mL

15.6-1000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 105% 93%-115%
Urine 93% 86%-101%
Human plasma 100% 81%-111%

Samplenmean (pg/mL)SDCV%
1 20 632.6 28.2 4.5
2 20 81.2 3.9 4.8
3 20 18.5 0.9 4.9

Samplenmean (pg/mL)SDCV%
1 24 659.6 62.0 9.4
2 24 83.3 4.5 5.5
3 24 15.5 0.3 2.0

Product overview:
KE00002 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TGF-β1 ELISA kit is to be used to detect and quantify protein levels of endogenous TGF-β1. The assay recognizes human TGF-β1. A polyclonal antibody specific for TGF-β1 has been pre-coated onto the microwells. The TGF-β1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for TGF-β1 is added to detect the captured TGF-β1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

CED, DPD1, LAP, TGF ?1, TGF beta 1, TGFB, TGFB1, TGFbeta

TGF-β is a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. TGF-β is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells. TGF-β acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. TGF-β plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. TGF-β appears to promote late stage progression and metastasis in some cancers.

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