Human TGF-beta1 ELISA Kit7 Publications

Catalog number: KE00002

规格

单价

96 T

¥2600

Images See all

Overview


Product name:
Human TGF-beta1 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants, Urine

Assay type:
Sandwich

Sensitivity:
3.3 pg/mL

Range:
15.6-1000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Urine 93% 86%-101%
Human plasma 100% 81%-111%
Cell culture supernatants 105% 93%-115%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 632.6 28.2 4.5
2 20 81.2 3.9 4.8
3 20 18.5 0.9 4.9

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 659.6 62.0 9.4
2 24 83.3 4.5 5.5
3 24 15.5 0.3 2.0

Product overview:
KE00002 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TGF-beta1 ELISA kit is to be used to detect and quantify protein levels of endogenous TGF-beta1. The assay recognizes human TGF-beta1. A monoclonal antibody specific for TGF-beta1 has been pre-coated onto the microwells. The TGF-beta1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for TGF-beta1 is added to detect the captured TGF-beta1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
CED, DPD1, LAP, TGF ?1, TGF beta 1, TGF- beta 1, TGFB, TGFB1, TGFbeta
Background

TGF-β is a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. TGF-β is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells. TGF-β acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. TGF-β plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. TGF-β appears to promote late stage progression and metastasis in some cancers.


Back
to top