Human IL4 ELISA Kit0 Publications

Catalog number: KE00016



96 T


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Product name:
Human IL4 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:

2.2 pg/mL

15.6-1000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Cell culture supernatant 95% 83%-105%
Human plamsa 103% 86%-116%

Samplenmean (pg/mL)SDCV%
1 20 777.0 45.1 5.8
2 20 225.5 6.4 2.8
3 20 43.3 2.4 5.5

Samplenmean (pg/mL)SDCV%
1 24 703.8 33.8 4.8
2 24 212.2 13.3 6.2
3 24 42.4 1.9 4.5

Product overview:
KE00016 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL4 ELISA kit is to be used to detect and quantify protein levels of endogenous IL4. The assay recognizes human IL4. A polyclonal antibody specific for IL4 has been pre-coated onto the microwells. The IL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL4 is added to detect the captured IL4 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4 + T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

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