Human CEA/CEACAM5 ELISA Kit

Sensitivity

4.6 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat No : KE00281

说明书下载

Synonyms

Carcinoembryonic antigen, CD66e, CEA, CEACAM5, DKFZp781M2392, Meconium antigen 100



Product Information

KE00281 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CEACAM5 ELISA kit is to be used to detect and quantify protein levels of endogenous CEACAM5. The assay recognizes human CEACAM5. An antibody specific for CEACAM5 has been pre-coated onto the microwells. The CEACAM5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for CEACAM5 is added to detect the captured CEACAM5 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human CEA/CEACAM5 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Saliva
Assay type Sandwich
Sensitivity 4.6 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 1048

Recovery

Sample TypeAverageRange
Serum107%96%-124%
Saliva114%105%-121%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120705.214.72.1
220171.32.71.6
32043.61.43.3

InterAssay

Samplenmean ( pg/mL)SDCV%
124683.430.54.5
224165.66.33.8
32436.02.46.7

Background Information

CEACAM5, also known as carcinoembryonic antigen (CEA) or CD66e, is a member of the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding. CEACAM5 is overexpressed in a wide variety of human cancers, including colon, breast, and lung. It inhibits anoikis and plays a role in tumorigenesis and metastasis. CEACAM5 is not usually present in the blood of healthy adults. However, it has been found that serum from people with colorectal, gastric, pancreatic, lung, and breast carcinoma has higher levels of CEACAM5 than healthy people. CEACAM5 is a tumor marker and is routinely exploited for diagnosis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Carcinoembryonic antigen, CD66e, CEA, CEACAM5, DKFZp781M2392, Meconium antigen 100