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Recombinant Human BCMA/TNFRSF17 protein (hFc Tag)

ED50

2-8 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

608

Accession

Q02223

Cat No : Eg0812

Print datasheet

Synonyms

BCM, BCMA, CD269, TNFRSF13A, TNFRSF17

验证数据展示

  • Purity of Recombinant Human BCMA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human BCMA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human BAFF (GST tag) at 1 μg/mL (100 μL/well) can bind Human BCMA (hFc tag) with a linear range of 2-8 ng/mL.

    Immobilized Human BAFF (GST tag) at 1 μg/mL (100 μL/well) can bind Human BCMA (hFc tag) with a linear range of 2-8 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human BAFF (GST tag) at 1 μg/mL (100 μL/well) can bind Human BCMA (hFc tag) with a linear range of 2-8 ng/mL.
Source HEK293-derived Human BCMA protein Met1-Ala54 (Accession# Q02223) with a human IgG1 Fc tag at the C-terminus.
Predicted Molecular Mass 32.4 kDa
SDS-PAGE 35-40 kDa
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • 12 months from the date of receipt, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

BCMA (B cell maturation antigen), also known as TNFRSF17, is 20.2-kDa type III transmembrane glycoprotein and is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes and plasma cells, which may be important for B cell development and autoimmune response (PMID: 32943087; 9846698). BCMA has two agonist ligands: a proliferation-inducing ligand (APRIL) and BAFF. When BCMA binds to APRIL, it transmits signals of cell survival and proliferation (PMID: 27127303); when BCMA binds to BAFF, it mediates the activation of NF-kappaB and MAPK8/JNK (PMID: 36140254). It has been found that the overexpression and activation of BCMA are associated with multiple myeloma (MM) in preclinical models and humans, supporting its potential utility as a therapeutic target for MM (PMID: 32055000; 32943087).

References:

1. Bo Yu et al (2020). J Hematol Oncol. Sep 17;13(1):125.
2. C Madry et al (1998). Int Immunol. Nov;10(11):1693-702.
3. Yu-Tzu Tai et al (2016). Blood. Jun 23;127(25):3225-36.
4. Fabrice Jardin (2022). Biomedicines. Sep 1;10(9):2153. 
5. Nina Shah et al (2020). Leukemia. Apr;34(4):985-1005.