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CoraLite® Plus 488-conjugated HuD-specific Polyclonal antibody

HuD-specific Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-24992

Print datasheet

Synonyms

ELAV like protein 4, ELAVL4, Hu antigen D, HUD, PNEM

验证数据展示

  • IF Staining of PC-12 using CL488-24992

    IF Staining of PC-12 using CL488-24992

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 HuD-specific antibody (CL488-24992) at dilution of 1:200.

经过测试的应用

Positive IF detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 24992-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24992 targets HuD-specific in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen HuD-specific fusion protein Ag21830 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
Synonyms ELAV like protein 4, ELAVL4, Hu antigen D, HUD, PNEM
Calculated Molecular Weight 380 aa, 42 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC036071
Gene Symbol ELAVL4
Gene ID (NCBI) 1996
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26378
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELAVL4, also named as HuD, is a 380 amino acid protein, which belongs to the RRM elav family. ELAVL4 is expressed in brain and localizes in the nucleus. ELAVL4 may play a role in neuron-specific RNA processing and protects CDKN1A mRNA from decay by binding to its 3'-UTR. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HuD-specific antibody CL488-24992Download protocol
Standard Protocols
Click here to view our Standard Protocols